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| Asymmetric Hydroxylation of 1,2,3,4- Tetrahydroquinoline by Hydroxylase-catalyzed Hydroxylation in Two-phase Reaction System |
| LI Ke， YANG Min， CUI Bao-dong, CHEN Yong-zheng |
| School of Pharmacy, Zunyi Medical University, Zunyi 563000, China |
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Abstract (R)-4-hydroxy-1,2,3,4-tetrahydroquinoline was synthesized by hydroxylation of 1,2,3,4-tetrahydroquinoline using the whole-cell of Pseudomonas plecoglossicidas ZMU-T02 as biocatalyst. The structure was confirmed by 1H NMR, 13C NMR, HR-MS and HPLC. Under the optimized reaction conditions［cell density 30 g cdw·L-1, pH 9.0, 50%(V/V) hexadecane as co-solvent］, when substrate concentration was 10 mmol·L-1, the yield and ee were 37% and 98%, respectively.
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Received: 06 January 2016
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2016, Vol. 24 
