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| Asymmetric Synthesis of (S)-(+)-4-phenyl-2-butanol by Carbonyl Reduction with Microbial Enzymes |
| YANG Min, LI Ke, ZHOU Xiao-jian, CUI Bao-dong, CHEN Yong-zheng |
| School of Pharmarcy, Zunyi Medical University, Zunyi 563000, China |
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Abstract Pseudomonas monteilii TA-5 cultivated with LB medium was employed as the biocatalysts（Cat) for the synthesis of (S)-(+)-4-phenyl-2-butanol with high enantioselectivity by asymmetric reduction. In this paper, the optimal reaction conditions for bioreduction of 4-phenyl-2-butanone（1） were optimized. Under the optimized reaction conditions［1 10 mmol·L-1, c(Cat) 30 g·cdw·L-1, 10% glycerol as the cosolvent, pH 8.0, 300 r·min-1, at 30 ℃ for 4 h］, the conversion was 82% and the ee was 91%.
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Received: 29 December 2015
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2016, Vol. 24 
